It can be difficult to keep up, and even specialists in one area of proteomics sometimes have difficulties applying their knowledge in other specialized areas.
New technologies and variations appear on what can seem to be a daily basis. Proteomics abounds with jargon and acronyms. As is the case for any emerging scientific field, proteomics makes a lot of sense to those performing large-scale protein analysis on a day-to-day basis, and much less sense to those looking in from the outside. It is from proteins that we shall learn how living cells and organisms are built and maintained, and how they fail when things go wrong. Genes are the instruments of change over evolutionary timescales, but proteins are the molecules that define which changes are accepted and which are discarded. If genes are the instruction carriers, proteins are the molecules that execute those instructions. Genes have sequences, but proteins have sequences, structures, biochemical and physiological functions, and their activities are influenced by chemical modification, localization within or without the cell, and perhaps most importantly of all, their interactions with other molecules.
The reason for this is the richness of proteomics data. The field of proteomics has grown rapidly in a short time, yet promises to provide more information about living systems than even the genomics revolution that started ten years before. It seeks to achieve what other large-scale enterprises in the life sciences cannot: a complete description of living cells in terms of all their functional components, brought about by the direct analysis of those components rather than the genes that encode them. Proteomics is the global analysis of proteins. Preface Proteomics, a word in use for less than a decade, now describes a rapidly growing and maturing scientific discipline, and a burgeoning industry. Two-dimensional gel electrophoresis three dimensional position specific scoring matrix affinity chromatography adenosine diphosphate atomic force microscopy adenosine triphosphate bicinchoninic acid Bacille Clamette-Guérin basic local alignment search tool class, architecture, topology and homologous superfamily calmodulin-binding protein charge-coupled device circular dichroism complementary DNA circular dichroism spectroscopy capillary electrophoresis chromatofocusing capillary gel electrophoresis 3-1propanesulfonate chalcone synthase collision-induced dissociation correlation spectroscopy complementary RNA direct analysis of large protein complexes DNA database of Japan dihydrofolate reductase difference gel electrophoresis deoxyribonucleic acid double-stranded RNA dithiothreitol N, N' dimethylaminopropylethylcarbodiimide epidermal growth factorĮGFR EGTA eIF ELISA EM ER ESI EST FMN fnII FRET FT-ICRĮpidermal growth factor receptor ethylene glycol-bis-(2-aminoethyl)-N, N, N', N' tetraacetic acid eukaryotic initiation factor enzyme-linked immunosorbent assay electron microscopy endoplasmic reticulum electrospray ionization expressed sequence tag falvin mononucleotide fibronectin type II domain fluorescence resonance energy transfer Fourier transform ion-cyclotron resonance 1.3 The genome, transcriptome and proteomeġ.4 Functional genomics at the DNA and RNA levelsĢ.2 Protein separation in proteomics-general principlesĢ.3 Principles of two-dimensional gel electrophoresisĢ.3.1 General principles of protein separation by electrophoresisĢ.3.2 Separation according to charge but not mass-isoelectric focusingĢ.3.3 Separation according to mass but not charge-SDS-PAGEĢ.4 Two-dimensional gel electrophoresis in proteomicsĢ.4.4 The representation of proteins on 2D-gelsĢ.5 Principles of liquid chromatography in proteomicsĢ.5.1 General principles of protein and peptide separation by chromatographyĢ.6 Multidimensional liquid chromatographyĢ.6.1 Comparison of multidimensional liquid chromatography and 2DGEĢ.6.2 Strategies for multidimensional liquid chromatography in proteomicsĪbbreviations 2DGE 3D-PSSM AC ADP AFM ATP BCA BCG BLAST CATH CBP CCD CD cDNA CDS CE CF CGE CHAPS CHS CID COSY cRNA DALPC DDBJ DHFR DIGE DNA dsRNA DTT EDC EGF
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